1103名体检人群不同糖代谢状况与红细胞压积、血小板相关性分析

目的:探讨干部人群中不同糖代谢状况与血红细胞压积、血小板的相关性。方法:根据不同糖代谢状态将血糖分层为血糖正常(NGT)、空腹血糖受损(IFG)、糖耐量减低(IGT)、IFG合并IGT、新诊断糖尿病(DM)5组,分析不同年龄、不同性别各组异常检出率,比较各组红细胞压积、血小板的变化,及其与糖代谢指标及各种病史的相关性。结果:1)男性糖代谢异常(包括IGT、DM、IFG)检出率高于女性;在各年龄组中,糖耐量减低的发生率明显高于空腹血糖受损。2)在糖耐量减低的233例中,50~59岁年龄组所占比例最高。3)DM组红细胞压积较NGT组明显升高,差异有统计学意义(P0.05),DM组、IFG合并IGT组血小板较NGT组明显升高,IFG合并IGT组血小板较IGT组明显升高,差异有统计学意义(P0.05)。4)多因素有序Logostic回归显示:年龄、体质指数、HCT、TG、高血压与糖代谢相关。结论:红细胞压积、血小板与糖尿病的发病密切相关。     

Relationship between obesity, glucose tolerance, and periodontal disease in Japanese women: the Hisayama study

BACKGROUND: Recent studies have reported a relationship between obesity and periodontal disease. Obesity is the strongest risk factor for type 2 diabetes, which is, in turn, a risk factor for periodontal disease. An oral glucose tolerance test is necessary to diagnose diabetes; however, no study has examined the relationship between obesity and periodontal disease by taking oral glucose tolerance test results into consideration. METHODs: In all, 584 Japanese women aged between 40 and 79 years old, with at least 10 teeth, underwent health examinations. Body mass index, waist-hip ratio, body fat, and oral glucose tolerance test results were used as independent variables with known risk factors for periodontal disease. Mean probing pocket depth and mean attachment loss were used as the dependent variables. RESULTS: In all of the analyses, body mass index, body fat, and waist--hip ratio were significantly associated with the highest quintile of mean probing pocket depth, even when adjusted for oral glucose tolerance test results. In the multivariate analysis, the subjects with the highest quartile of body mass index had a significantly higher odds ratio (OR) for the highest quintile of mean probing pocket depth [OR, 4.3; 95% confidence interval (CI), 2.1--8.9; p<0.001], whereas neither impaired glucose tolerance nor diabetes were significantly associated with deep pockets. The relationships between the obesity indexes and mean attachment loss did not reach statistical significance. CONCLUSION: Obesity was associated with deep pockets in Japanese women, even after adjusting for oral glucose tolerance test.     

Detection of stable and active periodontitis sites by clinical assessment and gingival crevicular acute-phase protein levels

The aim of the present study was to investigate whether incipient periodontal disease breakdown could be associated with changes in gingival crevicular fluid (GCF) acute-phase protein levels. In addition, the potential of clinical indices to act as predictors of significant attachment level (AL) change was investigated. AL measurements were taken at baseline and 3 months using the Florida Probe stent handpiece from a total of 384 sites in 38 patients. The average standard deviation of duplicate AL measurements was 0.423. When the tolerance method was used to detect significant AL change, 3.9% of the sites lost attachment. When a less stringent criterion of AL change of ≥1 mm was used 9.9% of the sites lost attachment during the 3-month period. With the exception of probing depth, baseline clinical parameters failed to predict AL change. Fourteen active periodontitis sites that demonstrated significant attachment loss were paired to stable periodontitis sites within the same patient. The levels of four acute-phase proteins, namely α2-macroglobulin (α2-M), α1-antitrypsin (α1-AT), transferrin (TF) and lactoferrin (LF), and also albumin (Alb) were assessed in the same gingival crevicular fluid sample using sandwich ELISAs. Results were expressed either as ng/30 s and ng/μg Alb. Acute-phase protein levels in GCF failed to differentiate between active and stable periodontitis sites at baseline. In conclusion, the degree of gingival inflammation of the tissues adjacent to the crevice/pocket seems to influence the levels of protease inhibitors and iron-binding proteins in GCF to a greater extent than probing attachment loss.     

变形链球菌耐氟菌株与亲代菌株质子移位膜ATP酶活性的比较

目的:通过测定并比较变形链球菌耐氟菌株及其亲代菌株体内质子移位膜ATP酶的活性,以阐明耐氟菌株耐酸能力提高的原因。方法:将变链菌株Ingbritt及其耐氟突变株Ingbritt-FR通过甲苯处理,2个循环的液氮冷冻和37℃解冻,制成透性细胞。将透性细胞悬液加到含有10mmol/LMgSO4的50mmol/LTris-maleate测试缓冲液中(pH6.0),加温至37℃,再加入5mmol/LATP(pH6.0)起动反应。分别于10、20、60min取样,测定样品中水解ATP所释出的无机磷量。采用磷钼酸比色法在紫外分光光度计上进行比色分析(660nm),所得数据采用双因素方差分析。结果:在10、20和60min时,耐氟菌株H+-ATP酶活性分别为308.48、136.67和82.80μmolPi/g细胞干重/min,显著高于亲代菌株的相应酶活性:104.77,、64.69和30.70(P<0.01)。随时间推移,两类菌株的H+-ATP酶活性均逐渐降低,在酶的作用时间差别上有统计学意义(P<0.01)。结论:耐氟菌株ATP酶活性增高为其耐酸性增高的原因;H+-ATP酶活性及耐酸性的增高将会增加变链菌耐氟菌株的致龋潜能。     

口腔鳞癌P-gp、GST-π表达与化疗药物耐受性

目的 探讨P－糖蛋白（P－glycoprotein,P-gp)和谷胱甘肽S转移酶π（glutathione transferase-π，GST-π）表达与化疗药物耐受性的关系。方法 采用免疫组化过氧化物酶标记法检测口腔颌面部鳞癌和正常口腔粘膜组织样本中P－gp和GST-π的表达，结合MTT药敏检测结果分析。结果 P－gp和GST-π在恶性肿瘤中阳性表达率分别为57．1％和53．6％，在正常组织中未见表达；P－gp的表达与化疗药物总体耐受性有关；GST-π的表达与患者顺铂耐受性有关。结论 P－gp和GST-π的阳性表达提示患者有化疗药物耐受倾向。P－pg和GST-π检测对判断口腔颌面部恶性肿瘤化疗耐药性有指导意义。     

中间普氏菌内毒素对人牙周膜细胞增殖的时间效应和细胞周期的影响

目的 :观察中间普氏菌内毒素 (Pi)对离体培养的人牙周膜细胞增殖的时间效应和细胞周期的影响。方法 :采用细胞培养技术、噻唑盐比色测定法 (MTT)和流式细胞仪技术。结果 :MTT法结果表明 ,加入10 μg/mLPi内毒素 3h后 ,即明显抑制人牙周膜细胞的增殖 (P <0 .0 5 ) ,且抑制作用随时间的延长而增强。流式细胞仪结果表明 ,在 10 μg/mLPi内毒素作用后 6和 12h ,DNA合成前期细胞所占百分比 (G1% )有所增高 ,而DNA合成期细胞所占百分比 (S % )降低。作用 2 4h后S期细胞数量增加 ,G1期细胞数量下降。结论 :Pi内毒素对人PDLC有直接毒害作用 ,可能是内毒素抑制静止态的G1期细胞进入S期 ,从而抑制细胞的增殖。     

老年人高龋患者牙菌斑中变形链球菌临床分离株耐酸能力的研究

目的:分析老年人高龋患者牙菌斑中,变形链球菌临床分离株(血清型C)在酸性条件下生存的耐酸能力。方法:体外培养从老年人高龋患者、无龋者牙菌斑中分离的101种不同基因型变形链球菌株临床分离株(血清型C),检测菌株在不同的pH条件下的耐酸能力。结果:老年人高龋患者口腔中分离出的变形链球菌临床分离株耐酸能力明显高于无龋组,与国际参考菌株无明显的统计学差异。同时研究发现,在老年人高龋患者同一个体的口腔中,变形链球菌临床分离株耐酸能力存在明显差异。结论:老年人高龋患者牙菌斑中变形链球菌临床分离株(血清型C)的高致龋性,与其携带有耐酸能力强的菌株关系密切。     

The induction of oral tolerance to Actinomyces viscosus in mice

OBJECTIVES: To determine whether oral tolerance with the oral bacterium Actinomyces viscosus was inducible in mice. MATERIALS AND METHODS: Mice were intragastrically (i.g.) and then intraperitoneally (i.p.) immunized with heat-killed A. viscosus. A control group of mice received only saline. A delayed type hypersensitivity (DTH) response and the levels of isotype specific antibodies were assessed. Spleen cells from mice that were i.g. immunized with A. viscosus were transferred to A. viscosus-primed mice in vivo and in vitro. Furthermore, mice were i.g. immunized with saline or A. viscosus and then challenged i.p. with saline, A. viscosus, or Porphyromonas gingivalis. RESULTS: Intragastric immunization with A. viscosus suppressed both DTH and serum specific antibodies to A. viscosus. DTH suppression lasted until week 4, while serum immunoglobulin (Ig)A and both IgG and IgM specific antibody levels remained suppressed up to week 8 and 12 respectively. IgG specific antibody suppression was transferable. The DTH response and serum antibodies specific to A. viscosus were suppressed in mice after i.g. challenged with A. viscosus but not P. gingivalis. CONCLUSION: Mucosal presentation of A. viscosus in mice led to the suppression of immune response to this bacterium in an antigen-specific fashion. Tolerance of DTH response was short lived, while suppression of antigen-specific IgG antibodies in mucosally tolerized mice was long-lasting.     

焦虑抑郁情绪对冠心病患者心肺运动试验的影响

目的： 比较冠心病、冠心病合并焦虑和/或抑郁患者的心肺运动试验(cardiopulmonary exercise test, CPET)的核心指标,探讨焦虑和/或抑郁情绪障碍对冠心病患者心肺功能及运动耐量的影响。方法： 筛选255 例稳定 期冠心病患者及302 例无冠心病者作为受试者,根据焦虑、抑郁量表结果将其分为对照组(n=96)、焦虑组(n=35)、抑 郁组(n=36)、焦虑抑郁组(n=135)、冠心病组(n=91)、冠心病合并焦虑组(n=35)、冠心病合并抑郁组(n=34)、冠心病合 并焦虑抑郁组(n=95),比较各组CPET核心指标的差异。结果： 冠心病各组峰值摄氧量、无氧域、峰值代谢当量、峰 值心率与对照组相比显著降低,而峰值收缩压、心率储备显著升高(均P<0.05);其中,冠心病合并焦虑抑郁组以上指 标与冠心病组相比,差异均有统计学意义(均P<0.05);焦虑组、抑郁组、焦虑抑郁组与对照组相比,差异均无统计学 意义(均P>0.05)。结论： 焦虑合并抑郁的冠心病患者心肺功能及运动耐量下降显著;单纯焦虑或单纯抑郁的冠心病 患者心肺功能及运动耐量有下降趋势。单纯焦虑、抑郁、焦虑合并抑郁并不会对非冠心病对照人群的心肺功能及运 动耐量产生负性影响。